Cardarine (GW-501516) Review: Side Effects, Cycles & Where to Buy?

Updated on December 5, 2023
Muscular Man Cardarine GW501516 guide review

Cardarine, scientifically known as GW-501516 and popularly referred to as Endurobol, stands at the forefront of modern supplements. It is a selective agonist of the peroxisome proliferator-activated receptor delta (PPARδ), mainly employed for the treatment of dyslipidemia, a condition characterized by abnormal levels of lipids in the bloodstream.

The PPARδ receptor is intriguing due to its ability to reside in a wide variety of body tissues, including adipose tissue, liver, skin, brain, and notably, skeletal muscle. This widespread presence of the receptor means that there will be many impacts on the body, influencing everything from lipid metabolism to energy balance.

Cardarine’s discovery dates back to the early 1990s, with a collaboration between GlaxoSmithKline (GSK) and Ligand Pharmaceuticals. This partnership was instrumental in pioneering research focused on metabolic diseases and disorders, resulting in this SARM being discovered.

Cardarine Overview: Is it a SARM?

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In the fitness space, Selective Androgen Receptor Modulators (SARMs) have gained a lot of popularity. SARMs, similar to anabolic and androgenic agents, act selectively on tissues, which mitigate most of the negative side effects associated with anabolic steroids.

Unlike SARMs, Cardarine uniquely targets the PPAR-δ pathway and does not directly interact with androgen receptors in muscle and bone tissues. This difference makes is so that Cardarine is not a SARM.

Is Cardarine a legal compound?

The legality of consuming Cardarine (GW 501516) varies depending on where you are in the world. While it is generally permitted in most parts of the world, there are exceptions like Australia. In many cases, obtaining Cardarine requires a doctor’s prescription. Often labeled as a “Research Chemical” or “Not for Human Consumption,” Cardarine is not yet FDA-approved and its primary use is for research purposes.

Most get Cardarine as a “research chemical” because it is legal to sell, just not consume as a human. So technically speaking you could give it to your “lab rat” and be in the clear legally speaking.

How Does Cardarine Work?

Cardarine functions by selectively binding to the PPAR receptor. This binding initiates the formation of the PPAR-coactivator complex with PGC-1, leading to increased expression of proteins that facilitate energy dissipation, aiding in enhanced energy availability.

During periods of physical exercise or long-term fasting, the body relies on the fatty acid metabolism. In these scenarios, the levels of PPAR in the muscles rise. Cardarine’s activation of PPAR results in increased lipid catabolism within skeletal muscles, which improves energy efficiency and utilization.

More Facts About Cardarine:

Cardarine GW501516 sarms benefits

Cardarine, a compound known for its unique ability to influence fatty acid oxidation, has raised the interest of both the scientific community and fitness community. Unlike selective androgen receptor modulators (SARMs), Cardarine functions through the activation of the peroxisome proliferator activated receptor (PPAR), particularly the delta variant – proliferator activated receptor delta (PPARδ). This pathway is key to understanding how Cardarine boosts the metabolism of fatty acids and increases fat burning capacity, making it very popular.

Clinical data has highlighted Cardarine’s impact on blood glucose regulation, a finding that aligns with its role in improving insulin sensitivity. This has led to its consideration as a potential therapeutic target for conditions characterized by impaired glucose metabolism and insulin resistance. In studies involving control mice and obese rhesus monkeys, Cardarine has shown promising results in regulating metabolic functions and improving mitochondrial function. Mice treated with Cardarine displayed altered gene expression, particularly in genes related to fat metabolism and energy utilization.

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Cardarine’s influence extends to mitochondrial biogenesis, the process by which new mitochondria are formed in the cell, a crucial aspect of cellular energy production. This part of Cardarine’s mechanism of action suggests its use as exercise mimetics, where it could mimic the effects of physical exercise in the body, which leads to higher endurance levels.

With sports and athletic performance, Cardarine has been subjected to urine tests for drug tested athletes, especially given its ability to increase serum levels and enhance performance. Athletes who have tested positive for Cardarine have faced scrutiny under anti-doping regulations, which further shows the SARMs impact on physical performance.

The ongoing clinical trials and studies involving healthy volunteers provide valuable insights into Cardarine’s safety profile. The increased serum levels of key biomarkers observed in these trials have reinforced Cardarine’s reputation as a very powerful metabolic modulator. However, the need for further development and research is needed, especially in understanding long-term effects.

Cardarine GW-501516 stands at the intersection of metabolic health and athletic performance. Its ability to modulate key pathways related to fatty acid metabolism, glucose regulation, and mitochondrial function makes it a great supplement. However, the balance between leveraging its benefits and understanding its risks requires careful navigation, guided by ongoing research and clinical insights.

Cardarine Pros and cons


  1. Enhances Stamina and Endurance: One of the biggest benefits of Cardarine is its ability to boost stamina. This is particularly beneficial in sports, where endurance is critical. The enhancement in stamina is attributed to improved fatty acid oxidation in skeletal muscle, which is vital in longer physical activities.
  2. Cardiovascular Health Benefits: Cardarine helps improve cardiovascular health. By influencing lipid profiles in blood lipids and aiding in the management of metabolic syndrome, it adds on to better heart health. This aspect is especially important given the rise in cardiovascular disease.
  3. Accelerates Fat Burning: A key feature of Cardarine is its ability to boost the body’s fat burning capacity, leading to weight loss. This rapid fat oxidation not only aids in weight loss but also plays a significant role in energy metabolism during exercise training.
  4. Muscle Mass Preservation: In addition to burning fat and weight loss, Cardarine helps in preserving muscle mass. This is crucial for athletes and individuals engaged in regular physical exercise, as it ensures the maintenance of muscle health and strength.
  5. Promotes Muscle Fiber Development: Cardarine aids in the development of muscle fibers, especially those linked with endurance. This leads to improved overall muscle growth and a great for those involved in endurance sports.
  6. Liver and Kidney Protection: Preliminary research shows Cardarine offers protective effects on vital organs like the liver and kidneys. This could be a significant advantage, considering the health risks associated with liver and kidney damage.


  1. Delayed Onset of Results: One of the drawbacks of Cardarine is that it may take around two months to give optimal results. This delayed response requires patience and consistent use, which is bad for those seeking immediate results.
  2. Risks Associated with Overconsumption: Like many metabolic modulators, consuming too much Cardarine can lead to negative effects. Adhere to recommended dosages to avoid negative side effects.
  3. Potential Digestive Issues: Some individuals who have taken Cardarine experience digestive problems, such as constipation and stomach cramps, particularly when starting the cycle or adjusting doses.

While Cardarine gives several amazing benefits of boosting stamina, improving cardiovascular health, and enhancing fat burning and muscle preservation, also be aware of its potential side effects. These include the time taken to show results, the risks associated with overconsumption, and possible digestive issues.

Lab tests and results

Cardarine has demonstrated impressive results in enhancing the metabolic efficiency of skeletal muscle, backed by various studies. In one study involving healthy, physically active individuals, knee extensor exercises were used to assess the impact of prolonged exercise on skeletal muscles. The results were clear: there was a significant increase in the transcription and mRNA content of the transcription factor coactivator PGC-1α in skeletal muscles, a direct result of extended physical activity (Source).

Furthermore, a cycling study involving human subjects confirmed the beneficial effects of Cardarine. The study found a notable 24% increase in total lipid oxidation, attributed to the increased expression of FAT/CD36 and CPT I mRNA. This is a strong indicator of Cardarine’s role in enhancing fat metabolism, a critical factor for those engaged in endurance training and seeking improved sports performance (Source).

In another study involving bovine embryos, GW501516 (Cardarine) was administered and compared to the control group and those treated with 2-BP. The findings were remarkable: embryos treated with Cardarine hatched quicker and exhibited lower levels of reactive oxygen species (ROS) and apoptosis. This suggests that Cardarine not only accelerates development but also enhances cellular health (Source).

The activation of PPARδ by Cardarine led to improved PEPCK and CPT1 protein expression in the embryos, establishing a balance between lipolysis and fatty acid β-oxidation. Additionally, it was demonstrated that the PPAR-δ agonist effectively lowered plasma insulin levels at the right doses, without adversely affecting glycemic control. In obese mouse models, this agonist notably improved glucose tolerance and insulin sensitivity by reducing adiposity.

A two-week clinical study with healthy and moderately overweight human volunteers showedfurther proof of Cardarine’s efficacy. The study showed that synthetic Cardarine ameliorated dyslipidemia and glucose metabolism by lowering plasma insulin and reducing liver fat content. This is a very important finding, particularly for individuals managing conditions like metabolic syndrome.Interestingly enough, when Cardarine was combined with TNFα (a pro-inflammatory cytokine), there was a reduction in the expression of adiponectin.

This indicates an increase of the insulin receptor, suggesting PPARδ, activated by Cardarine, could be a viable treatment for insulin resistance in type 2 diabetes mellitus patients. Finally, studies have shown that the activation of PPAR-δ by Cardarine significantly attenuates hydrogen peroxide (H2O2)-induced expression of TSP-1 in vascular smooth muscle cells (VSMCs), thereby reducing cellular oxidative stress.

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Moreover, the increase in muscle PGC-1α, associated with Cardarine use, has been linked to reduced muscle atrophy and improved muscle function in cases of Duchenne Muscular Dystrophy (DMD).These studies collectively show how effective Cardarine is in enhancing metabolic functions, reducing oxidative stress, and potentially improving conditions related to muscle degeneration and metabolic disorders.

To purchase Cardarine, a great option is to go through Chemyo, known for its reliability and quality assurance. Amidst a market where many shady vendors offer this product at inflated prices and questionable quality, Science Bio is a superior choice for its commitment to authenticity and affordability.

Here, customers can acquire a bottle of Cardarine for $55.96. On top of that, the site also offersfree shipping when orders are over $100.

When it comes to shipping and delivery, Chemyo is very reliable and efficient. Within the United States, customers can expect their packages to arrive within a week following the shipment. International deliveries generally reach their destination within two weeks, although occasional delays can occur due to customs processes.

Chemyo offers a very transparent and user friendly approach to Cardarine, or other supplements, ensuring that each transaction is as risk-free as possible.

Does Cardarine cause cancer?

man shirtless doing dumbbell curls

Several recent studies have shown the potential of Cardarine in inhibiting cancer development. In particular, its role in promoting apoptosis (programmed cell death) in certain cancer cells has been a focus of research. For instance, in the undifferentiated C666-1 nasopharyngeal carcinoma (NPC) cells, Cardarine has been shown to induce apoptosis, which effectively prevents the emergence of new cancer cells. This property suggests that Cardarine may offer protection against certain types of cancer.

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On top of that, there has been significant research examining the interaction of Cardarine with peroxisome proliferator-activated receptor delta (PPARδ) in cancer cells. For instance, a study found that the administration of GW501516, in conjunction with PPARδ, led to a reduction in tumorigenicity in breast cancer cells of mice. This finding indicates that Cardarine, when acting through the PPARδ pathway, may have anti-tumorigenic properties.

On the other hand, some studies have raised concerns about the potential carcinogenic effects of Cardarine. Notably, a study linked PPARδ activation, which is a key mechanism of Cardarine, to the development of colitis-associated colorectal cancer in mice. Keep in mind that the exact mechanism through which this occurs remains unclear.

The interaction between PPARδ and c‐Myc in the presence of Cardarine has been studied. With this research, it was observed that PPARδ forms an antitumorigenic complex with c‐Myc when Cardarine is present. This complex formation could result in a protective effect against tumorigenesis, however, the full implications of this interaction are still being explored.

In short, Cardarine does not cause cancer.

Modulating Inflammatory Responses in Muscles

Cardarine plays a huge role in modulating the inflammatory response in muscles. Frequent muscle use occasionally leads to tissue damage and inflammation, which can degrade muscle recovery and functional ability. Cardarine’s ability to reduce inflammation is critical in enhancing muscle recovery and maintaining optimal muscle health.

Improving Insulin Sensitivity

One of the key benefits of Cardarine is its impact on insulin sensitivity. By activating PPARδ, it facilitates the burning of fatty acids, leading to improved insulin sensitivity in the liver, muscle, and adipose tissues. This is particularly beneficial in managing conditions like insulin resistance and metabolic syndrome.

On top of that, Cardarine influences the activity of PGC-1, which co-activates the glucocorticoid receptor and the liver-enriched transcription factor HNF-4α. This activation plays an important role in healthy hepatic gluconeogenesis, an essential process in maintaining balanced blood glucose levels.

Reduction of Inflammation in Skeletal Muscles

Findings suggest Cardarine’s ability to reduce the production of pro-inflammatory cytokines is a key benefit. This reduction in local inflammatory responses helps control tissue damage and restores muscle tetanic contraction. This is particularly beneficial for athletes, bodubuilders and those engaged in regular physical exercise, as it aids in quicker recovery and sustained muscle performance.

Improvement in Lipoprotein Metabolism

Another benefit of Cardarine is its positive effect on lipoprotein metabolism. It has been shown to increase levels of good cholesterol (HDL) and decrease levels of bad cholesterol (LDL). This double effect is crucial in managing cardiovascular risk factors and promoting overall cardiovascular health.

Enhancement of Running Endurance

In animal studies, particularly those involving mice, Cardarine has shown to enhance running endurance. This is attributed to its role in increasing serum levels of galactose and β-hydroxybutyrate, key factors in prolonged energy release during strenuous activities.

Beneficial Effects on Blood Vessels

Cardarine positively affects blood vessels by elevating nitric oxide levels, which relaxes and protects them. Additionally, it is known to reduce oxidative damage in blood vessels, which further adds to cardiovascular health.

Liver Protection

The liver, a central organ in fat regulation, benefits from Cardarine. Normally, the liver converts glucose into energy, however, Cardarine redirects this process, making the liver convert fatty acids into energy instead. This shift not only reduces unwanted fats in the body but also protects the liver from damage related to fat accumulation.

Reducing Lipid Accumulation in Adipose Cells

Finally, Cardarine ensures a lower accumulation of lipids in adipose cells. This leads to the normal production of adipocytokines and promotes the burning of fats in the liver and muscle, adding to overall metabolic health and helping with weight management.

Cardarine offers a vast number of benefits to enhancing physical performance and metabolic health. From improving insulin sensitivity to protecting vital organs, Cardarine is great compound in both medical and athletic circles.

Side effects of Cardarine

While Cardarine (GW-501516) has shown incredible results in various areas such as performance enhancement and metabolic health, understanding its side effects is crucial for informed usage.

Cancer Risks in Animal Studies

One of the biggest concerns raised about Cardarine is its link to cancer development, particularly highlighted in animal studies. Clinical trials conducted on mice revealed that high doses of Cardarine over prolonged periods led to the development of cancer. However, it’s important to note that the doses used in these studies were significantly higher than those recommended for human use. This finding necessitates further investigation and cautious interpretation when considering Cardarine’s safety profile.

Role in Killing Cancer Cells

Conversely, human studies have shown a different aspect of Cardarine’s interaction with cancer. It has been observed to play a role in promoting the death of cancer cells. When used within the proper dosage range, Cardarine does not seem to produce significant side effects, showing a complex relationship with cancer that varies between species and dosage levels.


Certain groups, such as pregnant women and men with low testosterone levels, are advised against using Cardarine. This precaution stems from the lack of comprehensive clinical data on these specific populations and the potential hormonal health risks.

Dermatological Concerns

Female users might experience skin and acne issues when using Cardarine. While these side effects are very minor, dosage and diet must still be given a lot of attention. Consulting with a nutritionist or healthcare provider can help lower these effects by tailoring the Cardarine plan to the individual’s specific needs.

Metabolic and Cardiovascular Implications

Cardarine’s influence on lipid metabolism and cardiovascular health also needs attention. While it has been shown to increase fat burning capacity and improve lipoprotein metabolism, its long-term effects on cardiovascular disease and blood lipids are still being studied. Users should be aware of these potential impacts, especially those with pre-existing cardiovascular conditions or metabolic abnormalities.

Endocrine and Muscular Effects

When it comes to endocrine health, the effects of Cardarine on insulin resistance and glucose uptake are of interest. Its potential to improve insulin sensitivity could be beneficial for individuals with metabolic syndrome. However, its impact on hormone levels, particularly in individuals with hormonal imbalances, remains an area for further research.

With skeletal muscle health, although Cardarine has shown benefits in skeletal muscle metabolism, the long-term effects on muscle tissue, especially in the context of exhaustive running performance and endurance training, are areas that require more research.

Recommendations for Use

Given the varying effects of Cardarine, users should approach its use with caution, considering both the potential benefits and risks. Follow recommended dosages and seek professional guidance, especially for those with pre-existing health conditions or those in sports.

While Cardarine offers several promising benefits, its side effects, particularly regarding long term effects and specific population groups, use it cautiously. More research and clinical studies will further clarify these aspects, leading to safer and more effective use of Cardarine.


Bodybuilder Big Muscles Bicep Curl

Natural bodybuilding is increasingly popular among both younger and middle-aged fitness influencers. Bodybuilders must finely tune their dietary habits, ensuring that calorie reduction leads to effective fat loss while preserving muscle mass. This balance is absolutely needed in achieving a desired physique along with strength.

Key Factors in Natural Bodybuilding

  1. Caloric Intake: Achieving lean body mass (LBM) requires a well-structured dieting approach. Research indicates that for effective weight loss while retaining LBM, the dieting period should extend beyond two to four months. Typically, bodybuilders can expect to lose about 0.5 to 1% of their body weight through this method, though this rate varies, especially among leaner individuals.
  2. Macronutrient Intake: Protein intake is pivotal for the growth and maintenance of lean body mass. Studies have shown that leaner individuals require a higher protein intake compared to those with higher body fat percentages. However, it’s also crucial to balance protein with adequate carbohydrate intake, as insufficient carbohydrates can lead to decreased performance and impaired strength training. Maintaining a higher protein-to-carbohydrate ratio is essential for preserving lean body mass and optimizing metabolic modulators.
  3. Individual Differences and Ketogenic Diets: The impact of ketogenic diets on bodybuilding is still an area ripe for further investigation. Preliminary studies, though limited in scope, suggest that ketogenic diets lead to an average reduction in calorie intake, which could influence body composition and energy metabolism.
  4. Timing of Meals: The timing of nutrient intake plays a significant role in natural bodybuilding. One study highlighted that consuming higher amounts of protein and carbohydrates before and after exercise led to gains in strength and muscle size, compared to supplements taken after training sessions. This underscores the importance of nutrient timing in relation to exercise training and muscle recovery.
  5. Frequency of Meals: The pattern of meal consumption also impacts bodybuilding outcomes. Irregular meal patterns were observed to decrease post-prandial thermogenesis, affecting insulin sensitivity and blood lipid profiles. Conversely, when athletes, such as boxers, consumed six meals a day, they experienced less loss in lean body mass compared to consuming the same diet in only two meals per day. These findings, detailed in studies (Study 1, Study 2), highlight the significance of meal frequency in maintaining lean body mass and enhancing athletic performance.

Natural bodybuilding is not easy, there are a lot of steps that go beyond simple exercise routines. It involves meticulous planning around caloric and macronutrient intake, individualized diet plans possibly including ketogenic diets, and strategic timing and frequency of meals. By focusing on these areas, bodybuilders can optimize their results, balancing fat loss with the preservation and growth of lean muscle mass.

Nutritional Supplements: A Key Component in Bodybuilding

The incorporation of nutritional supplements alongside resistance and cardiovascular training is commonplace. Cardarine has emerged as a popular supplement in this regard, owing to its potential benefits in fat oxidation and endurance enhancement.

Micronutrients: Essential Yet Often Overlooked

While there is no definitive evidence linking micronutrient deficiencies directly to hindered bodybuilding progress, the importance of a balanced intake of certain micronutrients cannot be overstated. Vitamins and minerals such as vitamin D, calcium, zinc, magnesium, and iron are crucial for overall health and may be depleted due to constant dieting and rigorous training regimes. These micronutrients play vital roles in muscle function, bone health, and energy metabolism, making their adequate consumption pivotal for bodybuilders aiming to achieve peak physical condition.

Psychosocial Challenges in Bodybuilding

The journey to bodybuilding competitions is not just physically demanding but also psychologically challenging. Many athletes face psychosocial issues such as binge eating behaviors, particularly around competition times. Anxiety, mood swings, and irritability are commonly reported among competitors.

Open communication about these challenges is vital. Studies have highlighted that female bodybuilders often struggle with fears of gaining fat and find it challenging to control eating impulses. Conversely, male bodybuilders frequently express dissatisfaction with their muscle size, exhibiting obsessive behaviors and, in some cases, eating disorders like anorexia nervosa and bulimia nervosa. These psychosocial aspects underscore the importance of mental health support in the bodybuilding community.

Customer Reviews

Adam, Age: NA, United States

“Cardarine is currently one of the strongest endurance enhancers you can find. I can say this with confidence because I have tried other products like Stenabolic as well. Having been extensively used by bodybuilders, Cardarine has proved to be worth everything it has got. I observed that this product ensures to fulfill the claims it makes.”

Ed, Aged 39, United States

“I always complained of having metabolic issues and was not able to reduce unwanted fat no matter what ways I tried. But after consuming Cardarine for just 6 weeks, I could see remarkable changes in my body.”

Richy, Aged 42, UK

My muscle mass improved, and so did my energy levels. All the bad fat went down rapidly. I could say this with assurance because I have been observing this since I began consuming Cardarine.”

The Recommended Dosage of Cardarine: Cycles

Determining the optimal dosage of Cardarine is crucial for its safe and effective use. After extensive dosage trials, it has been established that a daily dose of 10 to 20 mg for approximately 8 weeks is effective. Notable improvements are often observed within the first 5 weeks. It’s advisable to start with a 10 mg dose and gradually increase to 20 mg, allowing the body to adapt.

While some athletes venture into higher dosages, up to 30 mg per day, caution is advised. The recommended maximum daily dosage is 20 mg, especially considering the limited human trials conducted with this compound. Overstepping this limit may increase the risk of potential side effects and health risks.

In conclusion, while nutritional supplements like Cardarine can play a significant role in enhancing bodybuilding performance, their use must be carefully balanced with a proper diet, mindful of micronutrient intake, and an understanding of the psychosocial aspects of bodybuilding. Additionally, adhering to recommended dosages and cycles of supplements such as Cardarine is crucial for maximizing benefits while minimizing risks.

Conclusion: Should You Take Cardarine?

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As we reach the conclusion of our in-depth exploration of Cardarine, it’s crucial to weigh its benefits and side effects based on the research and data available. Cardarine, a well known metabolic modulator, has been a subject of considerable interest in both the medical and athletic communities. Its ability to burn stored fat in skeletal muscles and utilize fatty acids for energy conversion leads to notable reduction in body fat, making very tempting.

Cardarine’s role in fatty acid oxidation is a key factor in its popularity among fitness fans and bodybuilders. By accelerating this metabolic process, it helps in the efficient use of energy resources, contributing to enhanced sports performance and endurance. This has made it a subject of interest to the World Anti Doping Agency, especially considering its potential for performance enhancement.

However, the use of Cardarine has not been without controversy. There have been concerns regarding drug caused cancer in clinical trials involving animals, although these findings have not been conclusively replicated in human studies. The lack of extensive clinical development and the limited number of clinical trials conducted on humans have left certain aspects, such as its long-term health risks and potential therapeutic targets, remains unclear.

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For individuals struggling with conditions like insulin resistance, Cardarine offers many benefits. Its ability to improve insulin sensitivity is huge, particularly considering the growing prevalence of metabolic disorders. However, it’s important to note that the activated receptor, peroxisome proliferator activated receptor (PPAR), through which Cardarine exerts its effects, has not been thoroughly studied in the context of long-term health implications.

Most classify Cardarine as a selective androgen receptor modulator (SARM). Cardarine actually does not fit this category despite many misconceptions. It operates through a different mechanism, targeting the PPAR pathway rather than directly interacting with androgen receptors.

Cardarines impact on blood glucose levels and blood lipids also adds to its appeal, particularly for those aiming to manage their weight and improve their metabolic health. However, due to the limited data from human studies and a lack of consensus among healthy volunteers, the exact effects on metabolic abnormalities is yet to be fully understood.

Do not overlook the ethical and legal considerations of using this compound, especially with sports. The absence of positive doping tests and its status under various anti-doping agencies necessitate caution for athletes considering its use. On top of that, the lack of data on the long-term effects of this supplement on mitochondrial function and the genes involved in its mechanism of action call for a wary approach.

To sum it all up, while Cardarine presents amazing benefits for fat loss, energy expenditure, and athletic performance, understand that there are potential side effects and risks. Until clinical trials and studies on treatment groups provides more solid answers, the use of Cardarine remains a personal choice, one that should be made with very careful consideration of all the existing evidence.

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