Laboratory Cleaning Checklist: What You Need

Updated on June 3, 2025
A close-up of a hand spraying water onto the white tile surface of a laboratory workplace while wearing blue latex gloves.

For any healthcare lab, maintaining cleanliness and sanitization is integral to the accuracy of lab results and the safety of staff. Sanitation directly impacts regulatory adherence, contamination prevention, and operational efficiency. In our guide below, we’ll explain what you need in your laboratory cleaning checklist, from essential cleaning supplies to disposal guidelines and more.

Essential Cleaning Supplies for Laboratories

A successful cleaning routine begins with the right tools and supplies. Laboratories require special equipment to clean and disinfect surfaces thoroughly. Common items for lab disinfection and sterilization include hydrogen peroxide, which is a powerful oxidizer that achieves high-level decontamination. Other common cleaning supplies include:

  • Autoclave-safe cleaning cloths
  • Chemical-resistant gloves
  • Spill containment kits
  • Eyewash solutions
  • HEPA-filtered vacuum cleaners

Selecting high-quality cleaning products ensures the effective neutralization of all bacteria, contaminants, and potential hazards.

Step-by-Step Laboratory Cleaning Procedure

Effective lab cleaning relies on a systematic approach. Start by clearing and decluttering workspaces to ensure cleaning accessibility. Next, use a disinfectant to sanitize surfaces such as benchtops, doorknobs, and equipment handles.

Be sure to adhere to disinfectant dwell times for maximum efficacy. Move on to cleaning large equipment, following manufacturer guidelines to prevent equipment damage. Finally, sweep and mop floors, focusing on corners and under equipment where contaminants often accumulate.

Safety Protocols To Follow During Cleaning

Safety must be a top priority during any lab cleaning procedure. Always wear appropriate personal protective equipment, including lab coats, gloves, goggles, and masks when necessary. Properly dispose of cleaning materials, especially those that encounter hazardous chemicals, to prevent exposure.

Ensure that everyone involved in cleaning has training in handling chemical spills, accidental exposure, and emergency protocols, including the use of eyewash stations and safety showers. Ventilation is also critical during cleaning. Use chemical fume hoods or ensure adequate air circulation to reduce inhalation risks from cleaning agents.

Disposal Guidelines for Laboratory Waste

Another thing you’ll need to add to your laboratory cleaning checklist is proper waste disposal. Segregate laboratory waste into categories such as biological, chemical, and general waste. Use containers with labels to differentiate these categories. Dispose of specific waste, like sharps, in puncture-proof containers to prevent injuries.

Chemical and biological waste often require treatment before disposal to neutralize risks. Partner with waste disposal services that comply with local and international regulations.

How Often You Should Clean the Lab

The frequency of cleaning depends on the type of lab and its activity level. High-use labs or those that handle hazardous chemicals may need daily cleaning for workspaces and equipment.

You should always clean floors daily to prevent the buildup of dirt and particulates. Conduct deep cleans weekly or monthly, focusing on less accessible areas and high-performance equipment. Tailoring cleaning schedules to the lab’s specific needs ensures it remains a safe and productive environment.

The Key to a Clean and Safe Lab

A clean laboratory is the foundation of accurate research, operational efficiency, and compliance with safety regulations. By following these steps, you set the stage for a safe, efficient, and contamination-free workspace.